Cell adhesion to the extracellular matrix (ECM) is essential for the development and maintenance of cells. Cell adhesion to various ECM as ligands is mainly mediated by cell surface receptor such as integrins. Human Collagen Type I is a key component in ECM for cell attachment and differentiation. M3 Peptide GEFYFDLRLKGDK that derived from human collagen type IV protein has been demonstrated in vitro as collagen mimetics and specific interacts with APP. This peptide will be a good coating matrix for cell attachment in serum free medium or enhance specific cell lineage differentiation.

Recombinant human AFPn-M3 peptide fusion protein was constructed by fusion human alpha fetal protein (AFP) N-terminal domain-I (1-198 aa) with human collagen type I derived GEFYFDLRLKGDK peptide. This protein was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant fusion protein at 5-10 ug / well (6 well plate) in a specific culture medium can be used for human red blood cells differentiation study in vitro.

1. As fusion protein fully derived from human protein, can be used as coating matrix protein to replace human collagen type IV for human cell differentiation regulation study in vitro.

2. Can be used in low serum or serum-free cell culture media to improve attachment and spreading of many normal and stem cells including human ES cell, endothelial, epithelial, hepatocytes and mesenchymal stem cells.

3. Can be used to improve survival of primary cultures.