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| Cata #: | Name of Product: | Price: |
| PRP-4376 | Recombinant sfGFP-ConA Fusion Protein | $150 |
| Product Name: | Recombinant sfGFP-ConA Fusion Protein |
| Catalog #: | PRP-4376 |
| Manufacture: | LD Biopharma, Inc. |
| Introduction: | Concanavalin A (ConA) is a plant mannose/glucose-binding lectin isolated from the seeds of cereal plants such as giant bean (Jack bean, Canavalia ensiformis), and its monomer can bind one Ca2+ and one Mn2+ which contains a glycosyl-binding site with high affinity for terminal α-D-mannosyl and α-D-glucosyl residues. ConA specifically binds to mannosyl- and glucosyl-containing extracellular glycoproteins, as such, allowing the ConA conjugated magnetic beads, or superGFP-CoA fusion protein, to effectively "grab" and hold onto these cells or nuclei through this carbohydrate interaction (CoA-beads for capturing cell or nuclei for CUT & RUN assay) or flow-cytometry using GFP-CoA as detection reagent.
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| Gene Symbol: | ConA |
| Accession Number: | AAB28242 |
| Species: | Canvalia Lineata ( Beach Bean) |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20% Glycerol in PBS buffer/1mM CaCl2 and 1mM MnCl2. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Fujimura,S., et al., Primary structures of concanavalin A-like lectins from seeds of twospecies of Canavalia. Phytochemistry 33 (5), 985-987 (1993) Li Li et al., Sample enrichment for single-nucleus sequencing using concanavalin A-conjugated magnetic beads. (2023) DOI: 10.1016/j.xpro.2023.102595 |
| Applications: | May be used for in vitro cells or nucleus isolation when combined with anti-GFP nano-antibody-magnetic beads. Also be used for flow-cytometry based assay for binding to cell surface glycoprotein. |
| Quality Control: | Purity: > 85 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKSGLRSGGSGGENLYFQGSEFADTIVAVELDTYPNTDIGDPSYPHIGIDIKSVRSKKTAKWNMQNGKVGTAHIIYNSVGKRLSAVVSYPNGDSATVSYDVDLDNVLPEWVRVGLSASTGLYKETNTILSWSFTSKLKSNSTHETNALHFVFNQFSKDQKDLILQGDATTGTDGNLELTRVSSNGSPQGNSVGRALFYAPVHIWESSAVVASFDATFTFLIKSSDSHPADGIAFFISNIDSSIPSGSTGRLLGLFPDAN |