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| Cata #: | Name of Product: | Price: |
| HRP-3271 | Recombinant Human CD13 Protein | $250 |
| Product Name: | Recombinant Human CD13 Protein |
| Catalog #: | HRP-3271 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Aminopeptidase N (ANPEP, also named as CD13) gene encodes a protein, which has broad specificity aminopeptidase. It plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. CD13 is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. CD13 may also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. It may have a role in angiogenesis and promote cholesterol crystallization. Recent data indicated that CD13acts as the receptor through which some enteric pathogens make their entry. And antibodies against CD13 receptor are known for their internalization across the mucosal surface through this receptor as potential mucosal vaccines development strategy. Full-length extracellular domain of human CD13 cDNA (33 – 967aa) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD13 (ANPEP; APN; PEPN) |
| Accession Number: | NP_001141.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Lisowska-Myjak B,et al., Can aminopeptidase N determined in the meconium be a candidate forbiomarker of fetal intrauterine environment? Exp Mol Pathol 115, 104446 (2020) Liu Z, et al., ACO2 and ANPEP as novel prognostic markers for gallbladder squamouscell/adenosquamous carcinomas and adenocarcinomas.Int J Clin Oncol 25 (7), 1346-1355 (2020) Qi F, et al., Single cell RNA sequencing of 13 human tissues identify cell typesand receptors of human coronaviruses. Biochem Biophys Res Commun 526 (1), 135-140 (2020) Li Z, et al., The human coronavirus HCoV-229E S-protein structure and receptorbinding. Elife 8, e51230 (2019) |
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| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGGEFELYSQEKNKNANSSPVASTTPSASATTNPASATTLDQSKAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK |