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| Cata #: | Name of Product: | Price: |
| HRP-3499 | Recombinant Human ECRG4 Protein | $250 |
| Product Name: | Recombinant Human ECRG4 Protein |
| Catalog #: | HRP-3499 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Esophageal cancer-related gene-4 (ECRG4, Augurin) gene encodes a probable hormone that may attenuate cell proliferation and induce senescence of oligodendrocyte and neural precursor cells in the central nervous system. ECRG4-induced senescence is characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation. ECRG4 is expressed in a wide variety of other cells/tissues, including cardiomyocytes and conduction system of the heart, the glomus cells of the carotid body, adrenal glands, choroid plexus, and leukocytes among others, where it exerts distinct functions, such as promoting/suppressing inflammation, inducing neuron senescence, stimulating the hypothalamus-pituitary-adrenal axis, maintaining the stemness of stem cells, participating in the rhythm and rate control of the heart, and possibly gauging the responsiveness of the cardiovascular system (CVS) to hypoxia. Full-length mature form (Augurin) of human ECRG4 cDNA (71-132aa) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | ECRG4 ( C2orf40 ) |
| Accession Number: | NP_115787 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Rui Zhou,. Potential functions of esophageal cancer-related gene-4 in the cardiovascular system. Front. Med., 2019, 13(6): 639-645 Dorschner RA, et al., ECRG4 regulates neutrophil recruitment and CD44 expression duringthe inflammatory response to injury. Sci Adv 6 (11), eaay0518 (2020) Zhou R, et al., Potential functions of esophageal cancer-related gene-4 in thecardiovascular system. Front Med 13 (6), 639-645 (2019) |
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| Quality Control: | Purity: > 92% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGQLWDRTRPEVQQWYQQFLYMGFDEAKFEDDITYWLNRDRNGHEYYGDYYQRHYDEDSAIGPR |