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HRP-3270 Recombinant Human COL3A1 C-terminal Protein $250

Recombinant Human COL3A1 C-terminal Protein

Product Name: Recombinant Human COL3A1 C-terminal Protein
Catalog #:  HRP-3270
Manufacture:  LD Biopharma, Inc.

Human Collagen type III protein (COL3A1) occurs in most soft connective tissues along with type I collagen. It is involved in regulation of cortical development. It is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. Naturally, 1466aa of precursor protein of Collage-III will be processed to N-terminal propetide (24-153aa) and mature collagen-III (154-1221aa) and its C-terminal domain (1222-1466aa) which also known as COLFI domain. The COL3A1 C-terminal domain have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

Full-length C-terminal domain (245aa) of human Collagen-III cDNA (1222 - 1466aa) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Accession Number:  NP_000081
Species:  Human
Package Size:  100 µg / Vial   
Composition: 2.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference:

Karayi AK, et al., Human skin fibrosis: up-regulation of collagen type III genetranscription in the fibrotic skin nodules of lower limb

Lymphedema. Trop Med Int Health 25 (3), 319-327 (2020)

Engqvist H, et al., Immunohistochemical validation of COL3A1, GPR158 and PITHD1 asprognostic biomarkers in early-stage ovarian carcinomas. BMC Cancer 19 (1), 928 (2019)

Kuivaniemi H et al., Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene 707, 151-171 (2019)

Jean-Marie Bourhis, et al., Structure basis of fibrillar collagen trimerization and related genetic disorders. Nat Struct Mol Biol. Oct;19(10): 1031-6.(2012)

  1. May be used for in vitro Collagen-III mediated cell attachment or receptor activation regulation study in vitro for various cells with this recombinant Collagen-III C-terminal domain protein either as soluble factor or as coating matrix protein. 
  2. May be used for COL3A1 C-terminal protein-protein interaction assay.
  3. Potential diagnostic protein, such as for monitoring ovarian carcinomas.
  4. As native human COL3A1 C-terminal domain antigen for specific antibody production.
Quality Control: Purity: > 93% by SDS-PAGE.
Recombinant Protein Sequence:


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