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HTF-3208 Recombinant Human TFEB-11R Protein $300

Recombinant Human TFEB-11R Protein

Product Name: Recombinant Human TFEB-11R Protein
Catalog #:  HTF-3208
Manufacture:  LD Biopharma, Inc.

Recent studies suggest that a number of genes encoding lysosomal proteins exhibit coordinated transcriptional behavior and are regulated by the transcription factor EB (TFEB). As transcription factor, TFEB specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. TFEB specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. TFEB plays a role in the signal transduction processes required for normal vascularization of the placenta.

Full-length human TFEB (475aa, Isoform-1. derived from BC032448) gene was constructed with codon optimized gene synthesis technology, and fusion with 28 aa N-terminal T7/His /TEV cleavage site Tag and 11 arginine (11R tag) at C-terminal. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Accession Number:  NP_009093
Species:  Human
Package Size:  40 µg / Vial   
Composition: 0.4 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT & Others.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks.
Key Reference:

Rushika M.Perera, et al., Transcriptional control of autophage-lysosome function drives pancreatic cancer metabolism. Nature. Vol 524, 361-365 (2015)

Magini A, et al., TFEB activation promotes the recruitment of lysosomalglycohydrolases beta-hexosaminidase and beta-galactosidase to theplasma membrane. Biochem. Biophys. Res. Commun. 440 (2), 251-257 (2013)

Song W, et al., TFEB regulates lysosomal proteostasis. Hum. Mol. Genet. 22 (10), 1994-2009 (2013)

Pastore N, et al., Gene transfer of master autophagy regulator TFEB results inclearance of toxic protein and correction of hepatic disease inalpha-1-anti-trypsin deficiency. EMBO Mol Med 5 (3), 397-412 (2013)

Hongyan Zhou, et al. Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins. Cell Stem Cell. 2009. 4: 381-384.

  • May be used for studying TFEB mediated autophagy–lysosome activation regulation for maintaining intracellular amino acid pools in vitro using intracellular protein delivery of recombinant TFEB-11R protein by adding protein directly into culture medium.
  • May be used for mapping TFEB protein-protein interaction.
  • May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
  • Highly purified human TFEB antigen for specific antibody development.
Quality Control: Purity: > 91 % by SDS-PAGE.
Recombinant Protein Sequence:


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