Human signal transducer and activator of transcription 3 (STAT3) gene encodes a signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once STAT3 activated, recruits co-activators, such as NCOA1 or MED1, to the promoter region of the target gene. It may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. STAT3 binds to the IL-6 responsive elements identified in the promoters of various acute-phase protein genes. It activated by IL31 through IL31RA. STAT3 acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells: deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. It is also involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. STAT3 mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation. It may play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macro-autophagy by inhibiting EIF2AK2/PKR activity. STAT3 plays a crucial role in basal beta cell functions, such as regulation of insulin secretion. 

Full-length human STAT3 cDNA (768aa, Isoform Del-701, derived from BC000627) was constructed with codon optimization gene synthesis and expressed with YFP Protein as N-terminal (YFP; 256aa) fusion protein in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Eroglu,M., et al., STAT3 expression is correlated with pathological stage in luminalsubtypes of breast carcinoma. Bratisl Lek Listy 121 (1), 51-61 (2020)

Caldenhoven E, et al.,STAT3beta, a splice variant of transcription factor STAT3, is adominant negative regulator of transcription. J. Biol. Chem. 271 (22), 13221-13227 (1996)

Akira S, et al., Molecular cloning of APRF, a novel IFN-stimulated gene factor 3p91-related transcription factor involved in the gp130-mediatedsignaling pathway. Cell 77 (1), 63-71 (1994)

1. May be used for in vitro STAT3 protein mediated gene transcription regulation for cancer cell study using intracellular delivery of recombinant human STAT3 protein with protein delivery reagent such as ProFectin. 
2. May be used for STAT3 protein-protein interaction assay.
3. May be used as specific substrate protein for STAT3 specific kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
4. Potential biomarker protein, for various cancer diagnosis / treatment applications.
5. As native human STAT3 immunogen for its specific antibody production.

MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNDLYKGSENLYFQGEFELAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM

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