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| Cata #: | Name of Product: | Price: |
| HTF-2084 | Recombinant Human NRF2 Protein | $250 |
| Product Name: | Recombinant Human NRF2 Protein |
| Catalog #: | HTF-2084 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human NRF2 gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. NRF2 binds to antioxidant response (ARE) elements in the promoter regions of target genes. It is important for the coordinated up-regulation of genes in response to oxidative stress. NRF2 may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. This proteins may also be involved in response to injury and inflammation which includes the production of free radicals. Full-length human NRF2 cDNA (604aa, Isoform-I, derived from BC011558) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | NRF2 ( NFE2L2; HEBP1; IMDDHH ) |
| Accession Number: | NP_006155.2 |
| Species: | Human |
| Package Size: | 30 µg / Vial |
| Composition: | 0.3 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Valerio Azzimato, et al., Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance. Science Translation Medicine,26:Feb.Vol.12,Issue 532,eaaw9709.(2020)
Yuan Z, et al., NRF2 overexpression in mesenchymal stem cells induces stem-cell marker expression and enhances osteoblastic differentiation Biochem. Biophys. Res. Commun. 491 (1), 228-235 (2017)
Vaamonde-Garcia C, et al., The nuclear factor-erythroid 2-related factor / heme oxygenase-1 axis is critical for the inflammatory features of type 2 diabetes-associated osteoarthritis. J. Biol. Chem. 292 (35), 14505-14515 (2017)
Perez-Leal O, et al., Pharmacological stimulation of nuclear factor (erythroid-derived 2)-like 2 translation activates antioxidant responses. J. Biol. Chem. 292 (34), 14108-14121 (2017) |
| Applications: |
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| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQEQLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGMQQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVGNCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN |