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| Cata #: | Name of Product: | Price: |
| HRP-3066 | Recombinant YFP-Human Tau Fusion Protein | $250 |
| Product Name: | Recombinant YFP-Human Tau Fusion Protein |
| Catalog #: | HRP-3066 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Microtubule-associated protein Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The Cterminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease, hyperphosphorylated Tau protein forms paired helical filaments (PHF) and straight filaments. Full-length human Tau cDNA (757aa, Isoform-I, derived from BC114948) was constructed with codon optimization gene synthesis and expressed with a YFP Protein Nterminal (YFP; 258aa) fusion at target protein N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | Tau (MAPTL; MTBT1) |
| Accession Number: | NP_058519 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Chojdak-Lukasiewicz J, et al., Plasma tau protein and Abeta42 level as markers of cognitive impairment in patients with Parkinson's disease Adv Clin Exp Med 29 (1), 115-121 (2020)
Sohn PD,et al., Pathogenic Tau Impairs Axon Initial Segment Plasticity and Excitability Homeostasis. Neuron 104 (3), 458-470 (2019)
Weingarten,M.D., et al., A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. U.S.A. 72 (5), 1858-1862 (1975) |
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| Quality Control: | Purity: > 92 % by SDS-PAGE. YFP protein: Ex λ = 517nm, and Em λ = 530nm. |
| Recombinant Protein Sequence: | MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNDLYKGSENLYFQGEFAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL |