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| Cata #: | Name of Product: | Price: |
| HRP-0523 | Recombinant Human CD166 Protein | $250 |
| Product Name: | Recombinant Human CD166 Protein |
| Catalog #: | HRP-0523 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Recent data indicated that CD166 expression levels were up-regulated in many tumor cells, and detection of CD166 could be used as biomarker for various disease diagnosis applications. Recombinant human CD166 extracellular domain cDNA (28 - 527 aa, Isoform 1) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 5-10 ug / well (6 well plate) in a specific culture medium may be used for human T and B cell cells activation/differentiation study in vitro. |
| Gene Symbol: | CD166 (ALCAM; MEMD) |
| Accession Number: | NP_001618 |
| Species: | Human |
| Package Size: | 100 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Minner,S., et al., Low activated leukocyte cell adhesion molecule expression is associated with advanced tumor stage and early prostate-specific antigen relapse in prostate cancer. Hum. Pathol. 42 (12), 1946-1952 (2011) Tachezy,M., et al., ALCAM (CD166) expression as novel prognostic biomarker for pancreatic neuroendocrine tumor patients. J. Surg. Res. 170 (2), 226-232 (2011) Smedbakken,L., et al., Activated leukocyte cell adhesion molecule and prognosis in acute ischemic stroke. Stroke 42 (9), 2453-2458 (2011) |
| Applications: | 1. Protein can be used as coating matrix protein for human T or B cell functions and differentiation regulation study in vitro. P 2. As potential biomarker protein for cardiovascular diseases and tumor diagnostic development. 3. As antigen for specific antibody production. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAK Download Datasheet |