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HRP-0523 Recombinant Human CD166 Protein $250

Recombinant Human CD166 Protein

Product Name: Recombinant Human CD166 Protein
Catalog #:  HRP-0523
Manufacture:  LD Biopharma, Inc.

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Recent data indicated that CD166 expression levels were up-regulated in many tumor cells, and detection of CD166 could be used as biomarker for various disease diagnosis applications.

Recombinant human CD166 extracellular domain cDNA (28 - 527 aa, Isoform 1) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 5-10 ug / well (6 well plate) in a specific culture medium may be used for human T and B cell cells activation/differentiation study in vitro.

Gene Symbol:  CD166 (ALCAM; MEMD) 
Accession Number:  NP_001618
Species:  Human
Package Size:  100 µg / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Minner,S., et al., Low activated leukocyte cell adhesion molecule expression is associated with advanced tumor stage and early prostate-specific antigen relapse in prostate cancer. Hum. Pathol. 42 (12), 1946-1952 (2011) Tachezy,M., et al., ALCAM (CD166) expression as novel prognostic biomarker for pancreatic neuroendocrine tumor patients. J. Surg. Res. 170 (2), 226-232 (2011) Smedbakken,L., et al., Activated leukocyte cell adhesion molecule and prognosis in acute ischemic stroke. Stroke 42 (9), 2453-2458 (2011)

1. Protein can be used as coating matrix protein for human T or B cell functions and differentiation regulation study in vitro. P

2. As potential biomarker protein for cardiovascular diseases and tumor diagnostic development.

3. As antigen for specific antibody production.

Quality Control: 1. Purity: > 90% by SDS-PAGE.
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