Human Thymocyte Selection-Associated High Mobility Group Box Protein TOX gene encodes a transcription factor. TOX protein contains a HMG box DNA binding domain. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. TOX function regulates T-cell development, especially in controlling CD8+ T cell exhaustion during chronically infection.

Full-length human TOX cDNA (525aa, derived from BC016665) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R) tag at its C-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

TOX                (  TOX1  )

Omar Khan, et al.,TOX trabscriptionally and epigenetically programs CD8+ T cell exhaustion. Nature. https://doi.org/10.1038/s41586-019-1325-x (2019) 

Lobbardi R,et al.,TOX Regulates Growth, DNA Repair, and Genomic Instability in T-cell Acute Lymphoblastic Leukemia. Cancer Discov 7 (11), 1336-1353 (2017)

Benoit BM, et al., CD164 identifies CD4(+) T cells highly expressing genes associated with malignancy in Sezary syndrome: the Sezary signature genes, FCRL3, Tox, and miR-214. Arch. Dermatol. Res. 309 (1), 11-19 (2017)

1. May be used for in vitro TOX mediated gene transcription regulation study in T cell cell’s differentiation by intracellular delivery of this TOX-11R protein directly in vitro cell culture.
2. May be used for mapping TOX protein-protein interaction.
3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
4. Potential biomarker protein / therapeutic target protein for T cell function related diseases, such as controlling T cell activities in vivo, et al.
5. As native Human TOX immunogen for its specific antibody production.

MASMTGGQQMGRGHHHHHHENLYFQGGEFDVRFYPPPAQPAAAPDAPCLGPSPCLDPYYCNKFDGENMYMSMTEPSQDYVPASQSYPGPSLESEDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSISVMPDIRNPEGTQYSSHPQMAAMRPRGQPADIRQQPGMMPHGQLTTINQSQLSAQLGLNMGGSNVPHNSPSPPGSKSATPSPSSSVHEDEGDDTSKINGGEKRPASDMGKKPKTPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPNATFGEVSKIVASMWDGLGEEQKQVYKKKTEAAKKEYLKQLAAYRASLVSKSYSEPVDVKTSQPPQLINSKPSVFHGPSQAHSALYLSSHYHQQPGMNPHLTAMHPSLPRNIAPKPNNQMPVTVSIANMAVSPPPPLQISPPLHQHLNMQQHQPLTMQQPLGNQLPMQVQSALHSPTMQQGFTLQPDYQTIINPTSTAAQVVTQAMEYVRSGCRNPPPQPVDWNNDYCSSGGMQRDKALYLTESGGGGSPGRRRRRRRRRRR

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