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| Cata #: | Name of Product: | Price: |
| HRP-2936 | Recombinant Human Meflin Protein | $250 |
| Product Name: | Recombinant Human Meflin Protein |
| Catalog #: | HRP-2936 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Meflin is a glycosylphosphatidyl inositol (GPI)-anchored protein encoded by the immunoglobulin superfamily containing leucine-rich repeat (ISLR/Islr) gene. Recently, it was reported that Mefilin is a specific marker of mesenchymal stromal/stem cells (MSCs) that are found in the perivascular space of multiple organs, including the bone marrow (BM). Meflin expression is limited to un-differentiated MSCs and is not detected in their differentiated lineages, such as mature osteoblasts, chondrocytes, and adipocytes. Positive cancer-associated fibroblasts was demonstrated to inhibit pancreatic carcinogenesis in xenograft tumor model. Full-length mature form of human Meflin cDNA (19 – 428aa) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | Meflin ( ISLR; HsT17563 ) |
| Accession Number: | NP_005536.1 |
| Species: | Human |
| Package Size: | 50µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Yasuyuki Mizutani, et al., Meflin-positive cancer-associated fibroblasts inhibit pancreatic carcinogenesis. Cancer Res. doi: 10.1158/0008-5472.CAN-19-0454. (2019) Maeda K, et al., Identification of Meflin as a Potential Marker for MesenchymalStromal Cells. Sci Rep 6, 22288 (2016) Nagasawa A, et al., Human and mouse ISLR (immunoglobulin superfamily containingleucine-rich repeat) genes: genomic structure and tissue expression. Genomics 61 (1), 37-43 (1999) |
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| Quality Control: | Purity: > 92 % by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGCPEPCDCGEKYGFQIADCAYRDLESVPPGFPANVTTLSLSANRLPGLPEGAFREVPLLQSLWLAHNEIRTVAAGALASLSHLKSLDLSHNLISDFAWSDLHNLSALQLLKMDSNELTFIPRDAFRSLRALRSLQLNHNRLHTLAEGTFTPLTALSHLQINENPFDCTCGIVWLKTWALTTAVSIPEQDNIACTSPHVLKGTPLSRLPPLPCSAPSVQLSYQPSQDGAELRPGFVLALHCDVDGQPAPQLHWHIQIPSGIVEITSPNVGTDGRALPGTPVASSQPRFQAFANGSLLIPDFGKLEEGTYSCLATNELGSAESSVDVALATPGEGGEDTLGRRFHGKAVEGKGCYTVDNEVQPSGPEDNVVIIYLSRAGNPEAAVAEGVPGQLPPGLLLLGQSLLLFFFLTSF Download Datasheet |