Human peptidase inhibitor 3; PI3 (also named as Elafin) gene encodes a secreted protein, which is neutrophil and pancreatic elastase-specific inhibitor of skin. PI3 may prevent elastase-mediated tissue proteolysis. PI3 functions as an anti-microbial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. Expression of this gene is up-gulated by bacterial lipopolysaccharides and cytokines.
PI3 was detected in its highest expression level in low esophagus mucosa.

Full-length mature form of human PI3 cDNA ( 61 – 117aa ) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Zhang W,et al.Expression and Clinical Significance of Elafin in Inflammatory Bowel Disease. Inflamm. Bowel Dis. 23 (12), 2134-2141 (2017)

Han W, et al., Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia. Int. J. Biochem. Cell Biol. 92, 173-182 (2017)

Wang T, et al., Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome: A Multicenter Survival and Longitudinal Prospective Observation Study. Shock 48 (2), 168-174 (2017)

Stalberg,C., et al., Anti-inflammatory Elafin in human fetal membranes
J Perinat Med 45 (2), 237-244 (2017)

1. May be used for in vitro PI3 mediated elastase-specific inhibitor study for various cells in vitro using this recombinant PI3 protein either as soluble factor or as coating matrix protein.
2. May be used for PI3 protein-protein interaction assay.
3. Potential therapeutic protein, which might be used for up-regulating local immune defense as effector function of gamma/delta T cells.
4. As native human PI3 immunogen for specific antibody production.

MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGGEAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ