Human Cadherin-13 gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer.

Full-length extracellular domain of human CDH13 gene (139-693 aa) was constructed with 31 N-terminal T7/His tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 1-10 ug / well (6 well plate) in neuronal cell specific medium can be used for human neuronal cell, vascular endothelial cell / receptor interaction study in vitro.

1. Protein can be used as coating matrix protein for study human neuronal and vascular endothelial cell / Recceptor interaction in vitro.

2. As highly purified protein, may be used as culture matrix protein for human neuronal axon connection study in vitro.

2. Functional Test: Not tested yet.