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| Cata #: | Name of Product: | Price: |
| HRP-2022 | Recombinant Human CT91-11R Protein | $300 |
| Product Name: | Recombinant Human CT91-11R Protein |
| Catalog #: | HRP-2022 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by human Ropporin-1A (ROPN1, also named as CT91) gene is found in the fibrous sheath of spermatazoa, where it interacts with rhophilin, a Rho GTPase binding protein. The CT91 can also bind an A-kinase anchoring protein (AKAP110) and a calcium-binding tyrosine phosphorylation-regulated protein (CABYR). Normally, this gene expression is restricted in human testis tissue, but it also expressed in a wide variety hematological tumor cells. As such, CT91 is a good candidate protein for anti-tumor vaccine development. Full-length mature human CT91 cDNA (211aa, derived from BC132744) was constructed with codon optimization gene synthesis and expressed with T7-His-TEV cleavage site (29aa Tag) at N-terminal and 11 arginine (11R tag) at C-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CT91 (ROPN1; ODF6; RHPNAP1; ROPN1a) |
| Accession Number: | NP_060048 |
| Species: | Human |
| Package Size: | 10 µg / Vial |
| Composition: | 0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Amer MK et al.,Ropporin gene expression in infertile asthenozoospermic men with varicocele before and after repair. Urology 85 (4), 805-808 (2015) Fiedler SE, et al., Loss of ASP but not ROPN1 reduces mammalian ciliary motility. Cytoskeleton (Hoboken) 69 (1), 22-32 (2012) Chiriva-Internati M, et al., Cancer testis antigen, ropporin, is a potential target for multiple myeloma immunotherapy. J. Immunother. 34 (6), 490-499 (2011) Salmaninejad A, et al. Cancer/Testis Antigens: Expression, regulation, Tumor Invasion, and Use in Immunotherapy of Cancers. Immunol Invest. Oct; 45(7): 619-640. (2016). Nicole Brooks, et al. Comparative Immunogenicity of a Cytotoxic T cell Epitope Delivered by Penetratin and TAT Cell Penetrating Peptides. Molecules. 20, 14033-14050. (2015) Madiha Derouazi, et al. Novel cell penetration peptide based vaccine induces robust CD4+ and CD8+ T cell mediated antitumor immunity. Cancer Res; 75(15) August 1, 3020-3031 (2015) |
| Applications: | 1. May be used for in vitro CT91 mediated anti-tumor immunotherapy for T cell activation study with this fusion protein for intracellular delivery in MHC-II pathway. 2. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 70% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFGAQTDKPTCIPPELPKMLKEFAKAAIRVQPQDLIQWAADYFEALSRGETPPVRERSERVALCNRAELTPELLKILHSQVAGRLIIRAEELAQMWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGSPRIPFSTFQFLYTYIAKVDGEISASHVSRMLNYMEQEVIGPDGIITVNDFTQNPRVQLEESGGGGSPGRRRRRRRRRRR Download Datasheet |