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HRP-2394 Recombinant Human CD54 Protein $300

Recombinant Human CD54 Protein

Product Name: Recombinant Human CD54 Protein
Catalog #:  HRP-2394
Manufacture:  LD Biopharma, Inc.

Human intercellular adhesion molecule-1 (ICAM-1, also named as CD54) proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, CD54 engagement promotes the assembly of endothelial apical cups through ARHGEF26 / SGEF and RHOG activation. CD54 acts as a receptor for major receptor group rhinovirus A-B capsid proteins. It also acts as a receptor for Coxsackievirus A21 capsid proteins. Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, it is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.

Full-length secreted form of human CD54 cDNA (28 – 480aa, derived from BC015969) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  CD54 (ICAM1; BB2) 
Accession Number:  NP_000192
Species:  Human
Package Size:  20 µg / Vial   
Composition: 0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Yan M, et al., Endothelial cell SHP-2 negatively regulates neutrophil adhesion and promotes transmigration by enhancing ICAM-1-VE-cadherin interaction. FASEB J. 31 (11), 4759-4769 (2017)
Raffray L, et al., Increased levels of soluble forms of E-selectin and ICAM-1 adhesion molecules during human leptospirosis. PLoS ONE 12 (7), e0180474 (2017)
Schaefer A, et al., Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro. J. Immunol. 198 (12), 4823-4836 (2017)
Viac J, et al., Epidermotropism of T cells correlates with intercellular adhesion molecule (ICAM1) expression in human papillomavirus (HPV)-induced lesions. J. Pathol. 168 (3), 301-306 (1992)

1. May be used for in vitro CD54 mediated endothelial/cardiomycyte interaction, differentiation regulation study with this protein either as soluble factor or as coating matrix protein.

2. May be used for protein-protein interaction assay.

3. Potential therapeutic/diagnostic protein, which may be used as matrix (recombinant CD54 or anti-CD54 antibody) for endothelial - cardio-repair/regeneration treatment.

4. As immunogen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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