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| Cata #: | Name of Product: | Price: |
| HTF-2096 | Recombinant Human Runx3-11R Protein | $300 |
| Product Name: | Recombinant Human Runx3-11R Protein |
| Catalog #: | HTF-2096 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human runt-related transcription factor 3 (Runx3) gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Recent data indicated that Runx3 can mediate TCF7 repression, which coordinately enforce acquisition of CT8+ TEFF (CT8 cytotoxic cell) functions and protect the cytotoxic lineage integrity by preventing TFH-lineage deviation. Full-length human Runx3 cDNA (428aa, Isoform-I, BC013362) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R tag) at its C-terminal. This Rnux3-11R fusion protein. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | Runx3 (AML2; CBFA3; PEBP2aC) |
| Accession Number: | NP_001026850 |
| Species: | Human |
| Package Size: | 20 µg / Vial |
| Composition: | 0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | J.Justin Milner, et al., Runx3 programs CD8+ T cell residency in non-lymphoid tissues and tumours. Nature doi: 10.1038/nature24993. (2017) Selvarajan V, et al., RUNX3 is oncogenic in natural killer/T-cell lymphoma and is transcriptionally regulated by MYC. Leukemia 31 (10), 2219-2227 (2017) Morita K, et al., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7), 2815-2828 (2017) Saikia S, et al., Alteration in the expression of MGMT and RUNX3 due to non-CpG promoter methylation and their correlation with different risk factors in esophageal cancer patients. Tumour Biol. 39 (5), 1010428317701630 (2017) |
| Applications: | 1. May be used for in vitro Runx3 mediated gene transcription regulation study of CD8+ T cell non-lymphoid or tumor tissue migration controlling by intracellular delivery of this protein. 2. May be used for mapping protein-protein interaction. 3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays. 4. Potential biomarker/therapeutic protein for cancer immune-therapy, such as regulating CD8 T cell tumor infiltration. 5. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFASNSIFDSFPTYSPTFIRDPSTSRRFTPPSPAFPCGGGGGKMGENSGALSAQAAVGPGGRARPEVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKLEDQTKPFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTSELNPFSDPRQFDRSFPTLPTLTESRFPDPRMHYPGAMSAAFPYSATPSGTSISSLSVAGMPATSRFHHTYLPPPYPGAPQNQSGPFQANPSPYHLYYGTSSGSYQFSMVAGSSSGGDRSPTRMLASCTSSAASVAAGNLMNPSLGGQSDGVEADGSHSNSPTALSTPGRMDEAVWRPYESGGGGSPGRRRRRRRRRRR Download Datasheet |