The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. Inter-alpha-inhibitor (IaI) complex has been associated to inflammation processes, hepatitis, cancer and even kidney diseases. The ITI family contains multiple proteins made up of a light chain and a variable number of heavy chains. Human inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) may act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan, which are essential to cells undergoing biological processes.

Full-length mature form of human IITIH2 cDNA (55 - 702aa) was constructed with codon optimization gene synthesis and expressed with N-terminal-T7-His-TEV cleavage site Tag (29aa) fusion in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro ITIH2 mediated hyaluronan and patrix protein intercation pathway regulation study in various cells with this chain protein either as soluble factor or as coating matrix protein.

2. May be used for protein-protein interaction assay.

3. As immunogen for specific antibody production.