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| Cata #: | Name of Product: | Price: |
| HRP-2286 | Recombinant Human CD301 Protein | $200 |
| Product Name: | Recombinant Human CD301 Protein |
| Catalog #: | HRP-2286 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human CD301 (also named as CLEC10A) encodes a C-type lectin domain family 10 member A protein, which probable role in regulating adaptive and innate immune responses. CD301 binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tumor-Antigen (Tn-Ag) and are expressed in a variety of carcinoma cells. Full-length extracellular domain of human CD301 cDNA (61 – 256aa, Isoform-III, derived from BC027858) was constructed with codon optimization gene synthesis and expressed with a human alpha Fetal Protein N-terminal (AFPn) -His-TEV cleavage site Tag (217aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD301 (CLEC10A; MGL; HML; CLECSF13) |
| Accession Number: | Q8IUN9-3 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Gaur P, et al., Intermediate monocytes are increased in enthesitis-related arthritis, a category of juvenile idiopathic arthritis. Clin. Exp. Immunol. 187 (2), 234-241 (2017) Lenos K, et al., MGL ligand expression is correlated to BRAF mutation and associated with poor survival of stage III colon cancer patients Oncotarget 6 (28), 26278-26290 (2015) Zizzari IG, et al., The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS ONE 10 (7), E0132617 (2015) van Vliet SJ, et al., Human T cell activation results in extracellular signal-regulated kinase (ERK)-calcineurin-dependent exposure of Tn antigen on the cell surface and binding of the macrophage galactose-type lectin (MGL). J. Biol. Chem. 288 (38), 27519-27532 (2013) |
| Applications: | 1. May be used for in vitro CD301 mediated immune-modulator activities regulation study for Macrophage/T cells interaction with this protein either as soluble factor or as coating matrix protein. 2. May be used for protein-protein interaction assay. 3. Potential therapeutic/diagnostic protein, which may be used as immune-modulator (recombinant CD301 or anti-CD301 antibody) for various diseases. 4. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGQNSKFQRDLVTLRTDFSNFTSNTVAEIQALTSQGSSLEETIASLKAEVEGFRQERQAVHSEMLLRVQQLVQDLKKLTCQVATLNNNASTEGTCCPVNWVEHQDSCYWFSHSGMSWAEAEKYCQLKNAHLVVINSREEQVRASGTQFLRHVPFREMVLKLGRTLESSGSFQNDCHLCHTLRDLIGLSIQRNISKLLS Download Datasheet |