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| Cata #: | Name of Product: | Price: |
| HTF-2239 | Recombinant Human KLF6-11R Protein | $300 |
| Product Name: | Recombinant Human KLF6-11R Protein |
| Catalog #: | HTF-2239 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human KLF6 gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis. Recent data indicated that KLF6a-Ccl2b/CCR7 signaling pathway plays an imperotant role in regulation of hematopoietic stem and progenitor cell lodgement and expansion. Full-length human KLF6 cDNA (282aa) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R) Tag at its C-terminal. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | KLF6 (BCD1; COPEB; CPBP; ST12) |
| Accession Number: | NP_001291 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Yuanyuan Xue, et al. The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b. Develomental Cell. 42, 1-14 (2017) Wang K, et al., miR-4262 Promotes Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting KLF6 and KLF15. Oncol. Res. 25 (2), 277-283 (2017) Gao Y, et al., KLF6 Suppresses Metastasis of Clear Cell Renal Cell Carcinoma via Transcriptional Repression of E2F1. Cancer Res. 77 (2), 330-342 (2017) Wen PH, et al.,Kruppel-like factor 6 suppresses growth and invasion of hepatocellular carcinoma cells in vitro and in vivo.Int J Immunopathol Pharmacol 29 (4), 666-675 (2016) |
| Applications: | 1. May be used for in vitro KLF6 mediated gene transcription regulation study for tumor and HSD differentiation & proliferation by intracellular delivery of this protein. 2. May be used for mapping protein-protein interaction. 3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays. 4. Potential biomarker/therapeutic protein for cancer treatment or regulating human HSC expansion in vitro. 5. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFDVLPMCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHLESGGGGSPGRRRRRRRRRRR Download Datasheet |