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HRP-1972 Recombinant Human IL36A Protein $300

Recombinant Human IL36A Protein

Product Name: Recombinant Human IL36A Protein
Catalog #:  HRP-1972
Manufacture:  LD Biopharma, Inc.

Human Interleukin-33 (IL36A) is a cytokine that binds to and signals through the IL1RL2/IL-36R receptor, which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. It seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes upregulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by upregulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. It may play a role in pro-inflammatory effects in the lung.

Full-length mature form of human IL36A cDNA (6 – 158aa) was constructed with codon optimization gene synthesis and expressed with a human Alpha Fetal protein N-terminal-His-TEV cleavage site Tag (217aa) fusion at AFP N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  IL36A (FIL1E; IL1E; IL1F6) 
Accession Number:  NP_055255
Species:  Human
Package Size:  50 µg / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Ciccia F, et al., Interleukin-36alpha axis is modulated in patients with primary Sjogren's syndrome. Clin. Exp. Immunol. 181 (2), 230-238 (2015)
Higgins J, et al., IL-36alpha induces maturation of Th1-inducing human MDDC and synergises with IFN-gamma to induce high surface expression of CD14 and CD11c. Hum. Immunol. 76 (4), 245-253 (2015)
Conde J, et al., IL-36alpha: a novel cytokine involved in the catabolic and inflammatory response in chondrocytes. Sci Rep 5, 16674 (2015)

1. May be used for in vitro IL36A mediated NF-kappa-B and MAPK signaling pathways regulation study in various cells with this protein either as soluble factor or as coating matrix protein.

3. Potential anti- immune response target protein for treatment of various immuno-related diseases using IL36A blocking antibodies.

4. As immunogen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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