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| Cata #: | Name of Product: | Price: |
| HRP-2105 | Recombinant Human CD96 Protein | $200 |
| Product Name: | Recombinant Human CD96 Protein |
| Catalog #: | HRP-2105 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by human T-cell surface protein tactile (CD96) gene is a receptor, which may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. It promotes NK cell-target adhesion by interacting with PVR present on target cells. CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Full-length extracellular domain of human CD96 cDNA (22 – 519aa) was constructed with codon optimization gene synthesis and expressed with a human N-terminalT7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD96 (TACTILE) |
| Accession Number: | NP_937839 |
| Species: | Human |
| Package Size: | 20 µg / Vial |
| Composition: | 0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | William C Dougall., et al., TIGIT and CD96: new checkpoint receptor targets for cancer immunotherapy. Immunol Rev. Mar; 276(1): 112-120. (2017) Zhang,W., et al., Expressions of CD96 and CD123 in Bone Marrow Cells of Patients with Myelodysplastic Syndromes. Clin. Lab. 61 (10), 1429-1434 (2015) Chavez-Gonzalez A, et al., Expression of CD90, CD96, CD117, and CD123 on different hematopoietic cell populations from pediatric patients with acute myeloid leukemia Arch. Med. Res. 45 (4), 343-350 (2014) Eriksson EM, et al., Differential expression of CD96 surface molecule represents CD8(+) T cells with dissimilar effector function during HIV-1 infection. PLoS ONE 7 (12), E51696 (2012) Hosen N, et al., CD96 is a leukemic stem cell-specific marker in human acute myeloid Leukemia. Proc. Natl. Acad. Sci. U.S.A. 104 (26), 11008-11013 (2007) |
| Applications: | 1. May be used for in vitro CD96 mediated T lymphocyte or NK cell activities regulation study with this protein either as soluble factor or as coating matrix protein. 2. May be used for protein-protein interaction assay. 3. Potential therapeutic protein, which may be used as checkpoint inhibitor (anti-CD96 antibody) for various cancer therapy. 4. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFVWEKTVNTEENVYATLGSDVNLTCQTQTVGFFVQMQWSKVTNKIDLIAVYHPQYGFYCAYGRPCESLVTFTETPENGSKWTLHLRNMSCSVSGRYECMLVLYPEGIQTKIYNLLIQTHVTADEWNSNHTIEIEINQTLEIPCFQNSSSKISSEFTYAWSVENSSTDSWVLLSKGIKEDNGTQETLISQNHLISNSTLLKDRVKLGTDYRLHLSPVQIFDDGRKFSCHIRVGPNKILRSSTTVKVFAKPEIPVIVENNSTDVLVERRFTCLLKNVFPKANITWFIDGSFLHDEKEGIYITNEERKGKDGFLELKSVLTRVHSNKPAQSDNLTIWCMALSPVPGNKVWNISSEKITFLLGSEISSTDPPLSVTESTLDTQPSPASSVSPARYPATSSVTLVDVSALRPNTTPQPSNSSMTTRGFNYPWTSSGTDTKKSVSRIPSETYSSSPSGAGSTLHDNVFTSTARAFSEVPTTANGSTKTNHVHITGIVVNKPKDGM Download Datasheet |