Human Tyrosine protein kinase receptor (Tyro3) gene encodes a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. It regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and auto-phosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, Tyro3 interacts with PIK3R1 and thereby enhances PI3-kinase activity. It activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. It Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Tyro3 also acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope . It also acts as a receptor for ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

Full-length extracellular domain of human Tyro3 cDNA (41 – 429aa, derived from BC051756) was constructed with codon optimization gene synthesis and expressed with a human N-terminalT7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro Tyro3 mediated signal pathway regulation study for various cell survival, migration and differentiation with this protein either as soluble factor or as coating matrix protein.

2. May be used for protein-protein interaction assay.

3. Potential therapeutic protein, may be used as GAS6 pathway specific inhibitor for various cancer therapies.

4. As native immunogen for specific antibody production.