Human Acid-sensing ion channel 4 (ACCN4) gene encodes a probable cation channel with high affinity for sodium. It is membrane protein with two transmembrane domain and one extracellular domain. In vitro, has no proton-gated channel activity. ACC$ gene was mainly detected for its expression in pituitary gland and weakly expressed in brain, vestibular system and organ of Corti. It forms a homotrimer or teterotrimer with other ASIC proteins. Recent data indicated that during cell damage or stress, ACCN family membrane plays an important role in apoptosis pathway, so regulating ACCN protein function in vivo map provides a good target for targeting diseases therapy, such as stroke treatment.

Full-length extracellular domain of human ACCN4 protein cDNA (217 – 546aa) was constructed with codon optimization gene synthesis and expressed with a human N-terminal T7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro ACCN4 mediated sodium channel activation / apoptosis regulation study for various neuronal cells with this protein either as soluble factor or as coating matrix protein.

2. May be used for protein-protein interaction assay.

3. Potential stroke therapeutic protein, such as ACC4 function, to be neutralized for protection of neuronal cell apoptosis during stroke treatment.

4. As immunogen for specific antibody production.