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HRP-1925 Recombinant Human FBXO4 Protein $300

Recombinant Human FBXO4 Protein

Product Name: Recombinant Human FBXO4 Protein
Catalog #:  HRP-1925
Manufacture:  LD Biopharma, Inc.

Human FBXO4 gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. It promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. FBXO4 recognizes TERF1 and promotes its ubiquitination together with UBE2D1.

Full-length human FBXO4 cDNA (386aa) was constructed with codon optimization gene synthesis technology and expressed with N-terminal T7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  FBXO4 (FBX4) 
Accession Number:  NP_036300
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Atkin G, et al., F-box only protein 2 (Fbxo2) regulates amyloid precursor protein levels and processing. JBC. Mar 7; 289(10): 7038-48. (2014)
Chu X, et al., Alternative splicing variants of human Fbx4 disturb cyclin D1 proteolysis in human cancer. Biochem. Biophys. Res. Commun. 447 (1), 158-164 (2014)
Lee EK, et al., The FBXO4 tumor suppressor functions as a barrier to BRAFV600E-dependent metastatic melanoma. Mol. Cell. Biol. 33 (22), 4422-4433 (2013)
Barbash O, et al., Phosphorylation-dependent regulation of SCF(Fbx4) dimerization and activity involves a novel component, 14-3-3varepsilon. Oncogene 30 (17), 1995-2002 (2011)

1. May be used for in vitro FBXO4 mediated ubiquitin protein ligase complex ( SKP1-cullin-F-box ) pathway regulation study for various cells by intracellular delivery of this protein with protein-delivery reagent such as ProFectin Reagent Kit.

2. May be used for protein-protein interaction assay.

3. Potential diagnostic protein for monitoring tumor progresses.

4. As immunogen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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