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HRP-1921 Recombinant Human ALDOA Protein $300

Recombinant Human ALDOA Protein

Product Name: Recombinant Human ALDOA Protein
Catalog #:  HRP-1921
Manufacture:  LD Biopharma, Inc.

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Anti-ALDOA antibody has been detected in auto-immuno-disease’s patient blood sample.

Full-length human ALDOA1 cDNA (363 aa) was constructed with codon optimization and expressed with N-terminal T7-His-TEV cleavage site Tag (29aa) fusion. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  ALDOA (ALDA; NY-LU-1, GSD12; HEL-S-87p) 
Accession Number:  NP_000025
Species:  Human
Package Size:  50 µg / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Ali O, at al., Human lung cancer antiogens recognized by autologous antibodies: Definition of a novel cDNA derived from the tumor suppressor gene locus on chromosome 3p21.3. Cancer Research 58. 1034-1041. (1998)
Long F, et al., Role of aldolase A in osteosarcoma progression and metastasis: in vitro and in vivo evidence. Oncol. Rep. 32 (5), 2031-2037 (2014)
Izzo P, et al. Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing. Eur. J. Biochem. 174 (4), 569-578 (1988)
Rehbein-Thoner,M. et al., The changes in aldolase isoenzyme pattern during development of the human kidney and small intestine--demonstrated in organ extracts and tissue sections. Hoppe-Seyler's Z. Physiol. Chem. 358 (2), 169-180 (1977)

1. May be used for in vitro ALDOA mediated glycolytic enzyme pathway regulation study for human muscle cells or tumor cells by intracellular delivery of this protein with protein-delivery reagent such as ProFectin Reagent Kit.

2. May be used for protein-protein interaction assay.

3. Potential diagnostic protein for auto-immune diseases.

4. As immunogen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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