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HRP-1857 Recombinant Human VTN-LAMAa3 Fusion Protein $150

Recombinant Human VTN-LAMAa3 Fusion Protein

Product Name: Recombinant Human VTN-LAMAa3 Fusion Protein
Catalog #:  HRP-1857
Manufacture:  LD Biopharma, Inc.

Extracellular matrix (EMC) and growth factor signaling environments are part of the nature mechanism for regulating stem cell fate. These micro-environmental stimuli are processed through a veritable network of intracellular signaling pathway. Evidence to date suggests that understanding of interactions between these pathway in defined cell culture condition are critical in controlling cell fate in vitro in the development of cell based therapeutic applications. Laminins are high-molecular weight (>400kD) protein of the extracellular matrix. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration and adhesion. Currently many active domain (peptide) from various laminins have been identified, which provide a opportunity for protein – engineering for its production.

To develop a specific coating matrix protein for representing human laminin a3, two active peptide domain from human laminin a1, were joint together with small linker and then fusion with human vitronectin (62-398aa). As human vitronectin provides an excellent polystyrene surface binding capacity, this recombinant VTN-laminin peptide fusion might serve as a unique coating matrix for various stem cell differentiation applications in vitro.


Two active peptide fusion fragments of human laminin a1 (86aa) was further fused to human vitronectin protein (62- 398aa with flexible linker domain GGGS in between) to generate a fusion protein, Named as VTN- LAMAα3. This VTN-LAMAα3 peptide cDNA was constructed with codon optimization gene synthesis technology. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  None (Named as VTN-ColIVa3 by manufacture) 
Accession Number:  NP_000629 + (artificial synthetic protein)
Species:  Human
Package Size:  40 µg / Vial   
Composition: 0.4 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Guokai Chen, et al. Chemically defined conditions for human iPSC derivation and culture. Nature Methods. 8. 424-429 (2011).
Braam, S.R. et al. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin. Stem Cells 26, 2257–2265 (2008).
Umezawa K. et al. Conformational requirement on peptides to exert laminin’s activities and search for protein segments with laminin’s activities. Biopolymers. 92 (2):124 – 131, (2009).
Applications: When coated at 0.5 - 1 ug/ ml per cm2 and combined with chemically defined culture medium, this recombinant protein may be used as matrix protein to replace native laminin a3 for benefiting different primary human cell culture in vitro.
Quality Control: Purity: > 90% by SDS-PAGE.
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