Human homeobox protein SIX2 is a transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. It acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Six2 also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development, it plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, Six2 controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, Six2 acts to mediate HOXA2 control over the insulin-like growth factor pathway. Six2 may also be involved in limb tendon and ligament development.

Full-length human SIX2 cDNA (290aa) was constructed with codon optimization gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro SIX2 mediated gene transcription regulation for various cancer cells study by intracellular delivery of this protein with “ProFectin” reagent.

May be used for mapping SIX2 protein-protein interaction.

2. May be used as enzymatic substrate for various proteases.

3. Potential biomarker protein for tumor chemotherapy or prognosis.

4. As immunogen for specific antibody production.