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hRP-1859 Recombinant Human VTN-LAMAγ1 Fusion Protein $150

Recombinant Human VTN-LAMAγ1 Fusion Protein

Product Name: Recombinant Human VTN-LAMAγ1 Fusion Protein
Catalog #:  hRP-1859
Manufacture:  LD Biopharma, Inc.

Extracellular matrix (EMC) and growth factor signaling environments are part of the nature mechanism for regulating stem cell fate. These micro-environmental stimuli are processed through a veritable network of intracellular signaling pathway. Evidence to date suggests that understanding of interactions between these pathway in defined cell culture condition are critical in controlling cell fate in vitro in the development of cell based therapeutic applications. Laminins are high-molecular weight (>400kD) protein of the extracellular matrix. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration and adhesion. Currently many active domain (peptide) from various laminins have been identified, which provide a opportunity for protein – engineering for its production.

To develop a specific coating matrix protein for representing human laminin γ1, two active peptide ( KAFDITYVRLKF GGGS RNIAEIIKDI ) domain from human laminin a5, were joint together with small linker and then fusion with human vitronectin (62-398aa). As human vitronectin provides a excellent polystyrene surface binding capacity, this recombinant VTN-laminin peptide fusion might serve as a unique coating matrix for various stem cell differentiation applications in vitro.

Two peptide fusion fragment of human laminin γ1 (26aa) was further fused to human vitronectin protein ( 62- 398aa ) to generate a fusion protein, Named as VTN-LAMAγ1. This VTN-LAMAγ1 peptide cDNA was constructed with codon optimization gene synthesis technology. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  None (Named as VTN-LAMAγ1 by manufacture) 
Accession Number:  NP_000629 + (artificial synthetic protein)
Species:  Based on Human protein sequence
Package Size:  50 µg / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Guokai Chen, et al. Chemically defined conditions for human iPSC derivation and culture. Nature Methods. 8. 424-429 (2011).
Braam, S.R. et al. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin. Stem Cells 26, 2257–2265 (2008).
Kuratomi Y. et al. Laminin gamma 1 chain peptide, C-16 (KAFDITYVRLKF) , promotes migration, MMP-9 secretion, and pulmonary metastasis of B16-F10 mouse melanoma cells. Br J Cancer. Apr 8: 86(7): 1169-73 (2002).
Paivi Liesi. et al. Identifycation of a neurite outgrowth-promoting domain of laminin using synthetic peptides. FEBS Letters. DOI: 10.1016/0014-5793(89)81180-9 (1989).
Applications: When coated at 0.5 - 1 ug/ ml per cm2 and combined with chemically defined culture medium, this recombinant protein may be used as matrix protein to replace native lamininγ1 for benefiting different primary human cell culture in vitro.
Quality Control: Purity: > 90% by SDS-PAGE.
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