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HRP-0433 Recombinant Human CD276 (Extracellular) Protein $125

Recombinant Human CD276 (Extracellular) Protein

Product Name: Recombinant Human CD276 (Extracellular) Protein
Catalog #:  HRP-0433
Manufacture:  LD Biopharma, Inc.

The protein encoded by human CD276 gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29.

Full-length extracellular domain of human CD276 gene (29 - 466 aa) was constructed with 29 N-terminal T7/His tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 1-10 ug / well (6 well plate) in T cell specific medium can be used for human T cell / receptor interaction study in vitro.

Gene Symbol:  CD276 (4Ig-B7-H3; B7-H3; B7H3) 
Accession Number:  NP_001019907
Species:  Human
Package Size:  100 ug / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: I., et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat. Immunol. 2 (3), 269-274 (2001) Calabro,L., et al. Expression and regulation of B7-H3 immunoregulatory receptor, in human mesothelial and mesothelioma cells: immunotherapeutic implications. J. Cell. Physiol. 226 (10), 2595-2600 (2011)

1. Protein can be used as coating matrix protein for human T cell / receptor interaction study in vitro.

2. As highly purified recombinant antigen, it may be used as cancer biomarker for diagnosis application development.

Quality Control:

1. Purity: > 90% by SDS-PAGE.

2. Functional Test: Not tested yet.

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