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vRP-1866 Recombinant West Nile Viral NS1 Protein $300

Recombinant West Nile Viral NS1 Protein

Product Name: Recombinant West Nile Viral NS1 Protein
Catalog #:  vRP-1866
Manufacture:  LD Biopharma, Inc.

West Nile (WNV) is a member of the Flavivirus genus of the Flaviviridae family. The Flavivirus genus includes other important human pathogens such as yellow fever (YFV), Dengue vius (DENV), Japanese encephalitis (JEV), and tick borne encephalitis (TBEV). Flaviviruses are enveloped viruses with a single stranded, <11 kb, positive-sense RNA genome, which encodes a single long open reading frame (ORF), The virus enters the host cell by receptor mediated endocytosis. Upon internalization and acidification of the endosome, fusion of viral and vesicular membranes allows release of the genomic RNA into the cytoplasm, which serves as mRNA. Translation of the single ORF at the rough ER produces a large polyprotein that is cleaved co- and posttranslationally into the mature proteins. The N-terminal of the polyprotein encodes the three structural proteins (C-prM-E), followed by at least seven non-structural (NS) proteins (NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5). Viral NS1 is a glycosylated 48-kD protein that plays a role in both viral replication and immune evasion. Secreted soluble WNV NS1 is found in patient serum during acute illness and is used as a diagnostic indicator of acute WNV infection. High levels of sNS1 are associated with increased disease severity.

Full-length West Nile virus NS1 (351aa) encoding gene was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  West Nile Virus: NS1 
Accession Number:  P06935 (Uniprot)
Species:  West Nile Virus
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Lindenbach BD, et al. Flaviviridae: The viruses and their replication; Fields Virology. Vol.1 pp: 1101–1152. (2007)
Akey DL, et al. Structure-guide insights on the role of NS1 in flavivus infection; Bioassays. May: 37 (5): 489-494. (2015)
Young LB, et al. Last 20aa of the West Nile virus NS1 protein are responsible for its retention in cell and the formation of unique heat-stable dimers; J gen Virol. May: 96(pt5): 1042-1054. (2015)
Chao DY, et al. Nonstructural protein 1 specific immunoglobulin M and G antibody capture enzyme-linked immunosorbent assays in diagnosis of flaviviral infection in humans; J Clin Microbiol. Feb: 53(2): 557-566. (2015)
Applications: May be used for specific antibody production or diagnostic applications.
Quality Control: Purity: > 90% by SDS-PAGE.
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