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hRP-0432 Recombinant Human KPNA2 Protein $300

Recombinant Human KPNA2 Protein

Product Name: Recombinant Human KPNA2 Protein
Catalog #:  hRP-0432
Manufacture:  LD Biopharma, Inc.

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. Human karyopherin alpha 2 (RAG cohort, importin alpha 1) KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V (D) J recombination.

Full length recombinant human KPNA2 protein was constructed with N-terminal 15aa (T7) tag. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  KPNA2 
Accession Number:  NP_002257
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Lange,A., et al. Classical nuclear localization signals: definition, function, and interaction with importin alpha. J. Biol. Chem. 282 (8), 5101-5105 (2007)
Sekimoto,T., et al. Importin alpha protein acts as a negative regulator for Snail protein nuclear import. J. Biol. Chem. 286 (17), 15126-15131 (2011)
Teng,S.C., et al. Importin KPNA2, NBS1, DNA repair and tumorigenesis. J. Mol. Histol. 37 (5-7), 293-299 (2006)

1. As soluble active recombinant protein, may be used for human nuclei protein transportation study in vitro.

2. As immunogen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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