Human CD89 gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. CD89 interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for human CD89 gene.

Full-length extracellular domain of human CD89 cDNA (22 – 227 aa, derived from BC027953) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro CD89 mediated IgA-opsonized targets activation in inflammation pathway regulation, with this protein either as soluble factor or as coating matrix protein.

2. May be used for protein-protein interaction mapping.

3. Potential biomarker protein for monitoring IgA mediated inflammation pathway status.

4. As immunogen for specific antibody production.