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| Cata #: | Name of Product: | Price: |
| hTF-1761 | Recombinant Human NFIL3 Protein | $300 |
| Product Name: | Recombinant Human NFIL3 Protein |
| Catalog #: | hTF-1761 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by human Nuclear Factor Interleukin-3-regulated protein (NFIL3) gene is a transcriptional regulator that binds (G/A)TTTA(C/T)GTAA(C/T) as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 can protect pro-B cells from programmed cell death. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. Recent data indicated that NFIL3 plays a role in regulating differentiation of pathogenic Th17 cells and boosts the generation of protective Th1 cells via Erk1/2 and the transactivation of the IL-10 promoter by ROR-α. Full-length human NFIL3 cDNA (461 aa) was constructed with codon optimization gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (31aa) fusion at its N-terminal. This protein is expressed in E Coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | NFIL3 (E4BP4; IL3BP1; NF-IL3A) |
| Accession Number: | NP_005375.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Mauricio F, et al., Melatonin contributes to the seasonality of multiple sclerosis relapses. Cell. Vol 162. Issue 6. Pp: 1338-1352 (2015) Tamai S, et al., Neuroprotective role of the basic leucine zipper transcription factor NFIL3 in models of amyotrophic lateral sclerosis J. Biol. Chem. 289 (3), 1629-1638 (2014) Keniry M, et al., Survival factor NFIL3 restricts FOXO-induced gene expression in cancer. Genes Dev. 27 (8), 916-927 (2013) Zhao M, et al., E4BP4 overexpression: a protective mechanism in CD4+ T cells from SLE patients. J. Autoimmun. 41, 152-160 (2013) Tong X, et al., Recruitment of histone methyltransferase G9a mediates transcriptional repression of Fgf21 gene by E4BP4 protein. J. Biol. Chem. 288 (8), 5417-5425 (2013) |
| Applications: | 1. May be used for in vitro NFIL3 mediated gene transcription regulation study for Th1 & Th17 cells through Erk1/2 signaling pathway by intracellular delivery of this protein with ProFectin reagent. 2. May be used for mapping NFIL3 protein-protein interaction assay. 3. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays. 4. Potential biomarker protein for monitoring T cell functions in various auto-immuno-diseases, such as multiple sclerosis relapses. P>5. May be used for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFGSQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSACRRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG Download Datasheet |