Dynein is a motor protein (also called molecular motor or motor molecule) in cells, which converts the chemical energy contained in ATP into the mechanical energy of movement. Dynein transports various cellular cargo by "walking" along cytoskeletal microtubules towards the minus-end of the microtubule, which is usually oriented towards the cell center. Thus, they are called "minus-end directed motors," while kinesins, motor proteins that move toward the microtubules' plus end, are called plus-end directed motors. Human dynein light chain 4, axonemal (DNAL4) is a component of the dynein motor complex which involved in generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity.

Full-length recombinant human DNAL4 (463aa) gene was constructed with 15aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro DNAL4 related cellular cargo movement regulation study coating with intracellular protein delivery of this protein.

2. May be used as enzymatic subtrate protein for kinase and ubiquitin assay development.

3. May be used for mapping DNAL4 protein-protein internaction.

4. As antigen for specific antibody production.

Purity: > 90% by SDS-PAGE.

Suggested Coating Protocol
Standard coating was performed using 1ml PBS / well, which contains 5-10 ug protein / well) for incubating at 4°C overnight. After coating, remove PBS solution, the plate is ready for cell culture study.