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hRP-0787 Recombinant Human CDC123 Protein $300

Recombinant Human CDC123 Protein

Product Name: Recombinant Human CDC123 Protein
Catalog #:  hRP-0787
Manufacture:  LD Biopharma, Inc.

The human CDC123 - coding sequence was first isolated from a cDNA expression library as a suppressor of the temperature-sensitive mutation in rat cell line 3Y1tsD123, which arrests in G1 phase at restrictive temperature. The D123 gene is expressed in the cytosol of human and rat tissues, with expression in testis being highest. Eukaryotic initiation factor 2 (eIF2) is a central regulator of translational initiation in times of growth and times of stress, CDC123 was identified as one of key regulator for controlling eIF2 activity in CDC123-Chf-Gcd11 axis. Recent genomic-wide SNP mapping data indicated CDC123 gene locus strongly associated with susceptibility to type 2 diabetes.

Full-length human CDC123 (336 aa) gene was constructed with 15aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  CDC123 (C10orf7; D123) 
Accession Number:  NP_006014
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Bieganowski,P., et al., Cdc123 and checkpoint forkhead associated with RING proteins control the cell cycle by controlling eIF2gamma abundance. J. Biol. Chem. 279 (43), 44656-44666 (2004)
Imamura,M., et al., Genetic variants at CDC123/CAMK1D and SPRY2 are associated with susceptibility to type 2 diabetes in the Japanese population. Diabetologia 54 (12), 3071-3077 (2011)

1. May be used for in eIF2 regulation study with intracellular protein delivery of this protein.

2. As soluble/native protein, may be used as enzymatic substrate protein for kinase and ubiquitin assay development.

3. May be used for mapping CDC123-Chf-Gcd11 axis protein–protein interaction.

4. May be used as antigen for specific antibody development and potential biomarker development for type 2 diabetes diagnosis.

Quality Control: Purity: > 90% by SDS-PAGE.
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