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hRP-0833 Recombinant Human NXT1 Protein $300

Recombinant Human NXT1 Protein

Product Name: Recombinant Human NXT1 Protein
Catalog #:  hRP-0833
Manufacture:  LD Biopharma, Inc.

The protein encoded by human NTF2-related export protein 1 (NXT1) gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export.

Full-length human NXT1 (140 aa) gene was constructed with 15 aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  NXT1 (MTR2; P15) 
Accession Number:  NP_037380
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Katahira,J., et al., Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling. J. Biol. Chem. 277 (11), 9242-9246 (2002)
Black,B.E., et al., NXT1 is necessary for the terminal step of Crm1-mediated nuclear export. J. Cell Biol. 152 (1), 141-155 (2001)
Ossareh-Nazari,B., et al., RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro. Mol. Cell. Biol. 20 (13), 4562-4571 (2000)

1. May be used for in vitro NXT1 mediated target specific mRNA nuclear export pathway regulation study with intracellular delivery of this protein.

2. As soluble / native protein, may be used as enzymatic substrate protein for kinase and ubiquitin assay development.

3. May be used for mapping NXT1 protein-protein interaction.

4. May be used as antigen for specific antibody development.

Quality Control: Purity: > 90% by SDS-PAGE.
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