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HRP-0355 Recombinant Human CD14 (Extracellular) Protein $125

Recombinant Human CD14 (Extracellular) Protein

Product Name: Recombinant Human CD14 (Extracellular) Protein
Catalog #:  HRP-0355
Manufacture:  LD Biopharma, Inc.

Human CD14 (monocyte differentiation antigen CD14) is a 375 amino acid, phospholipid anchored cell surface protein. This protein is preferentially expressed on monocytes / macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide.

Full-length extracellular domain of human CD14 gene (20-345 aa) was constructed with 29 N-terminal T7/His tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 1-10 ug / well (6 well plate) in T cell specific medium can be used for human T cell / receptor interaction study in vitro.

Gene Symbol:  CD14 
Accession Number:  NP_000582
Species:  Human
Package Size:  100 ug / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Schumann,R.R.,et al. Structure and function of lipopolysaccharide binding protein, Science 249 (4975), 1429-1431 (1990) Feng,A.L., et al. CD16+ monocytes in breast cancer patients: expanded by monocyte chemoattractant protein-1 and may be useful for early diagnosis. Clin. Exp. Immunol. 164 (1), 57-65 (2011)

1. Protein can be used as coating matrix protein for study human T cell / Recceptor interaction in vitro.

2. As highly purified rotein, may be used as breast cancel biomarker for for diagnosis application when combined with CD16 antigen.

Quality Control:

1. Purity: > 90% by SDS-PAGE.

2. Functional Test: Not tested yet.

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