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| Cata #: | Name of Product: | Price: |
| HTF-0350 | Recombinant Human Glis1-11R Protein | $300 |
| Product Name: | Recombinant Human Glis1-11R Protein |
| Catalog #: | HTF-0350 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Glis1 (Zinc Finger protein GLIS1) encodes a member of the GLI (MIM 165220) related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription. Recent publication indicated that Glis1 plays a very important role in generation of induced pluripotent stem cells. Full length recombinant human Glsi1 protein was constructed with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in culture mediums at concentration of 2 – 8 ug/ml may be used for studying of human direct reprogramming of somatic cells. |
| Gene Symbol: | Glsi1 |
| Accession Number: | NP_671726 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days. |
| Key Reference: | Maekawa,M., et al. Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature 474 (7350), 225-229 (2011), Hongyan Zhou, et al. Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins. Cell Stem Cell. 4: 381-384 (2009). |
| Applications: | 1. Protein transduction for in vitro somatic cell reprogramming study. 2. Active recombinant protein, may be used for DNA / protein binding assay. 3. As specific protein substrate for MAP kinase assay. 4. As immunogen for specific antibody production. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. 2. DNA binding activity: Not tested yet. |
| Recombinant Protein Sequence: | 29aa_Tag_AEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGLPPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACRKASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDTLEESGGGGSPGRRRRRRRRRRR Download Datasheet |