Human E-Cadherin (CDH1) is an 882 amino acid protein (1-23 = signal domain) that belongs to a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Recent publication from Ding Sheng’s group indicated that coated recombinant human E-cadherin protein (extracellular domain) alone benefits human ES cell Culture.

Recombinant human E-Cadherin gene (155-710 aa Fragment) was constructed with codon optimization and expressed in non-fusion protein form in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 5-10 ug / well (6 well plate) in either NutriStem or Stempro medium may be benefit for human iPS cell generation in vitro.

1. Used as coating matrix protein for maintaining long-term ES or iPS cell culture then combine with proper ES cell culture mediums.

2. As excellent coating matrix materials for 11R tag recombinant TF intracellular delivery for protein derived iPS protocol with extremely low-level non-specific interaction.

3. As native antigen for antibody production.

1. Purity: > 90% by SDS-PAGE.

2. Functional Test: Each lot was tested for human ES cell (H1) culture using 5 – 10 ug in 1 ml Nutristem medium per well (6 well plate).

Coating Protocol

Use 1ml PBS per well, add 5 - 10µg protein to each well and incubate at 4°C overnight. After coating, remove PBS solution, the plate is ready for ES cell cultivation.