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| Cata #: | Name of Product: | Price: |
| hRP-1363 | Recombinant Human CD305 Protein | $200 |
| Product Name: | Recombinant Human CD305 Protein |
| Catalog #: | hRP-1363 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by human leukocyte-associated immunoglobulin-like receptor 1 (CD305) gene is an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. Human CD305 is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. Human CD305 gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. Recent data demonstrated that CD305 activities may be involved in systemic lupus disease development. Full-length human CD305 extracellular domain cDNA (22-165aa, derived from BC027899) was constructed with a human Alpha-Fetal Protein N-terminal domain (AFPn)-His-TEV cleavage site (219aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD305 (LAIR-1) |
| Accession Number: | NP_002278 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Colombo,B.M., et al., Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS ONE 7 (2), E31903 (2012) Rygiel,T.P., et al., Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR-1. Mol. Immunol. 49 (1-2), 402-406 (2011) Xue,J., et al., Leukocyte-associated immunoglobulin-like receptor-1 is expressed on human megakaryocytes and negatively regulates the maturation of primary megakaryocytic progenitors and cell line. Biochem. Biophys. Res. Commun. 405 (1), 128-133 (2011) |
| Applications: | 1. May be used for in vitro human CD305 mediated leukocyte regulation study with this protein as either coating matrix protein or soluble factor. 2. May be used for CD305 protein-protein interaction assay. 3. As potential diagnostic biomarker for systemic Lupus. 4. As antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGEFQEEDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERESRSTYNDTEDVSQASPSESEARFRIDSVSEGNAGPYRCIYYKPPKWSEQSDYLELLVKETSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEHLY Download Datasheet |