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| Cata #: | Name of Product: | Price: |
| hRP-0711 | Recombinant Human COASY Protein | $300 |
| Product Name: | Recombinant Human COASY Protein |
| Catalog #: | hRP-0711 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. Human bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes. Recent data indicated that this enzyme form complex with p85aPI3K , and plays a unique role in bladder cancer development. Full-length human COASY (269aa, Isoform_b) gene was constructed with 15 N-terminal T7 tag. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | COASY (DPCK; NBP; pOV-2; PPAT; UKR1) |
| Accession Number: | NP_001035996 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Daugherty,M., et al., Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. J. Biol. Chem. 277 (24), 21431-21439 (2002) Aghajanian,S.et al., Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase). Biochem. J. 365 (PT 1), 13-18 (2002) Breus,O., et al., CoA synthase is in complex with p85alphaPI3K and affects PI3K signaling pathway. Biochem. Biophys. Res. Commun. 385 (4), 581-585 (2009) |
| Applications: | 1. May be used for in vitro human COASY mediated biosynthesis of coenzyme A regulation study for various cells by intracellular delivery of this protein with “ProFectin” reagent. 2. May be used for mapping COASY protein-protein interaction. 3. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays. 4. COASY mRNA were enriched in CD105+ endothelial cells, so detection of this protein in blood sample may be used as specific bio-marker protein for monitoring endothelial cell functions. 5. May be used as antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGEFMAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD Download Datasheet |