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hRP-0711 Recombinant Human COASY Protein $300

Recombinant Human COASY Protein

Product Name: Recombinant Human COASY Protein
Catalog #:  hRP-0711
Manufacture:  LD Biopharma, Inc.

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. Human bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC and dephospho-CoA kinase (DPCK; EC, in prokaryotes. Recent data indicated that this enzyme form complex with p85aPI3K , and plays a unique role in bladder cancer development.

Full-length human COASY (269aa, Isoform_b) gene was constructed with 15 N-terminal T7 tag. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  COASY (DPCK; NBP; pOV-2; PPAT; UKR1) 
Accession Number:  NP_001035996
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Daugherty,M., et al., Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. J. Biol. Chem. 277 (24), 21431-21439 (2002)
Aghajanian, al., Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase). Biochem. J. 365 (PT 1), 13-18 (2002)
Breus,O., et al., CoA synthase is in complex with p85alphaPI3K and affects PI3K signaling pathway. Biochem. Biophys. Res. Commun. 385 (4), 581-585 (2009)

1. May be used for in vitro human COASY mediated biosynthesis of coenzyme A regulation study for various cells by intracellular delivery of this protein with “ProFectin” reagent.

2. May be used for mapping COASY protein-protein interaction.

3. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.

4. COASY mRNA were enriched in CD105+ endothelial cells, so detection of this protein in blood sample may be used as specific bio-marker protein for monitoring endothelial cell functions.

5. May be used as antigen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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