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| Cata #: | Name of Product: | Price: |
| HTF-0270 | Recombinant Human ZNF746 Protein | $300 |
| Product Name: | Recombinant Human ZNF746 Protein |
| Catalog #: | HTF-0270 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human ZNF746 (PARIS) is a 644 amino acid protein that contains a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues. Recent data indicated that ZNF746 accumulated in models of parkin inactivation and in human Parkinson’s disease (PD). ZNF746 represses the expression of the transcriptional co-activator, PGC-1a and the PGC-1a target gene, NRF-1 by binding to insulin response sequences in the PGC-1a promoter. Meanwhile, ZNF746 is also regulated by E3 ubiquitin ligase (parkin) and play its role in the progress of PD disease. Full-length human ZNF746 cDNA (643aa, derived from BC068505) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | ZNF746 (PARIS) |
| Accession Number: | NP_689770 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg / ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT, Glycerol and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Joo-Ho Shin., et al. PARIS (ZNF746) repression of PGC-1a contributes to neurodegeneration in Parkinson’s disease. Cell.144. 689-792 (2011)
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| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFAEAVAAPISPWTMAATIQAMERKIESQAARLLSLEGRTGMAEKKLADCEKTAVEFGNQLEGKWAVLGTLLQEYGLLQRRLENVENLLRNRNFWILRLPPGSKGESPKEWGKLEDWQKELYKHVMRGNYETLVSLDYAISKPEVLSQIEQGKEPCNWRRPGPKIPDVPVDPSPGSGPPVPAPDLLMQIKQEGELQLQEQQALGVEAWAAGQPDIGEEPWGLSQLDSGAGDISTDATSGVHSNFSTTIPPTSWQTDLPPHHPSSACSDGTLKLNTAASTEDVKIVIKTEVQEEEVVATPVHPTDLEAHGTLFGPGQATRFFPSPAQEGAWESQGSSFPSQDPVLGLREPARPERDMGELSPAVAQEETPPGDWLFGGVRWGWNFRCKPPVGLNPRTGPEGLPYSSPDNGEAILDPSQAPRPFNEPCKYPGRTKGFGHKPGLKKHPAAPPGGRPFTCATCGKSFQLQVSLSAHQRSCGAPDGSGPGTGGGGSGSGGGGGGSGGGSARDGSALRCGECGRCFTRPAHLIRHRMLHTGERPFPCTECEKRFTERSKLIDHYRTHTGVRPFTCTVCGKSFIRKDHLRKHQRNHAAGAKTPARGQPLPTPPAPPDPFKSPASKGPLASTDLVTDWTCGLSVLGPTDGGDM |