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| Cata #: | Name of Product: | Price: |
| HRP-1456 | Recombinant Human CD273 Protein | $150 |
| Product Name: | Recombinant Human CD273 Protein |
| Catalog #: | HRP-1456 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human programmed cell death 1 ligand 2 (PD-L2, CD273) is a cell membrane protein, which involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Recombinant human CD273 extracellular domain cDNA (20-220 aa, derived from BC074766) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD273 (PD-L2; PDCD1LG2; B7DC; bA574F11.2; Btdc) |
| Accession Number: | NP_079515.2 |
| Species: | Human |
| Package Size: | 100 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Messal,N., et al,. PD-L2 is expressed on activated human T cells and regulates their function. Mol. Immunol. 48 (15-16), 2214-2219 (2011)
Du,G.H., et al., The high expression level of programmed death-1 ligand 2 in oral lichen planus and the possible costimulatory effect on human T cells. J. Oral Pathol. Med. 40 (7), 525-532 (2011) Singh,A.K., et al,. Role of PD-L1 and PD-L2 in allergic diseases and asthma. Allergy 66 (2), 155-162 (2011) |
| Applications: | 1. May be used for in vitro CD273 mediated T cell differentiation regulation study with this protein as either coating matrix protein or soluble factor. 2. May be used for protein-protein interaction assay. 3. Potential biomarker protein for clinical monitoring T cell function in vitro. 4. As antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGEFLFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT Download Datasheet |