Invention in the REGENERATIVE MEDICINE is what we do every day, benefits of patients is our final goal

Our Products

Home > Products

Cata #: Name of Product: Price:
HRP-1321 Recombinant Human PD1 Protein $150

Recombinant Human PD1 Protein

Product Name: Recombinant Human PD1 Protein
Catalog #:  HRP-1321
Manufacture:  LD Biopharma, Inc.

Human programmed cell death protein 1 (PD-1, CD279) gene encodes a cell surface membrane protein of the immunoglobulin superfamily. CD279 protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for CD279 gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that CD279 gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Full-length length human PD1 extracellular domain cDNA (21-170aa, derived from BC074740) was constructed with codon optimization and expressed with human Alpha-Fetal Protein (AFP) N-terminal domain Tag (1-198 aa) fusion at PD1 N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  PD1 (PDCD1; hPD-1; CD279; SLEB2) 
Accession Number:  NP_005009.2
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Fourcade,J., et al., CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72 (4), 887-896 (2012)
Wiesmayr,S., et al., Decreased NKp46 and NKG2D and elevated PD-1 are associated with altered NK-cell function in pediatric transplant patients with PTLD. Eur. J. Immunol. 42 (2), 541-550 (2012)
Li,M., et al., HBcAg induces PD-1 upregulation on CD4+T cells through activation of JNK, ERK and PI3K/AKT pathways in chronic hepatitis-B-infected patients. Lab. Invest. 92 (2), 295-304 (2012)

1. May be used for in vitro pro-B and T cells differentiation regulation study with this protein as either coating matrix protein or soluble factor.

2. May be used for protein-protein interaction assay.

3. As potential diagnostic biomarker protein for monitoring NK, B and T cell activities in vivo.

?4. As antigen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
Download Datasheet