Invention in the REGENERATIVE MEDICINE is what we do every day, benefits of patients is our final goal
| Cata #: | Name of Product: | Price: |
| HRP-1266 | Recombinant Human UCHL1 Protein | $300 |
| Product Name: | Recombinant Human UCHL1 Protein |
| Catalog #: | HRP-1266 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The protein encoded by Human ubiquitin carboxyl-terminal hydrolase isozyme L1 ( UCHL1) gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. Full-length mature form of human UCHL1 cDNA (1 – 220 aa, derived from BC005117) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | UCHL1 (NDGOA; PARK5; PGP9.5; PGP95) |
| Accession Number: | NP_004172 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Sucrose. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Schroder,C., et al., Prognostic relevance of ubiquitin C-terminal hydrolase L1 (UCH-L1) mRNA and protein expression in breast cancer patients. J. Cancer Res. Clin. Oncol. 139 (10), 1745-1755 (2013)
Fellenberg,J., et al., Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells. Cancer Lett. 336 (1), 61-67 (2013) Kabuta,T., et al., Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. J. Biol. Chem. 288 (18), 12615-12626 (2013) |
| Applications: | 1. May be used for in vitro UCHL1 protein mediated neuronal cell differentiation regulation study with “ProFectin” based intracellular delivery of this protein. 2. May be used for UCHL1 protein – protein interaction assay. 3. As Enzymatic substrate for various proteases. 4. Potential diagnostic biomarker protein for various cancer diseases. 5. May be used for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHENLYFQGGEFQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALC Download Datasheet |