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| Cata #: | Name of Product: | Price: |
| HRP-1151 | Recombinant Human KNG1 Heavy Chain Protein | $150 |
| Product Name: | Recombinant Human KNG1 Heavy Chain Protein |
| Catalog #: | HRP-1151 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Kininogen-1 (KNG1) gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Full-length heavy chain domain (HMWK) of human KNG1 cDNA (19 – 380 aa, derived from BC060039) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | KNG1 (BDK; BK; KNG) |
| Accession Number: | NP_000884 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Hatoh,T., et al., Domain 5 of high molecular weight kininogen inhibits collagen-mediated cancer cell adhesion and invasion in association with alpha-actinin-4. Biochem. Biophys. Res. Commun. 427 (3), 497-502 (2012)
Gutierrez-Venegas,G.et al., Bradykinin promotes TLR2 expression in human gingival fibroblasts. Int. Immunopharmacol. 11 (12), 2079-2085 (2011) Kolte,D.,et al., High molecular weight kininogen activates B2 receptor signaling pathway in human vascular endothelial cells. J. Biol. Chem. 286 (28), 24561-24571 (2011) |
| Applications: | 1. May be used for in vitro KNG1 H chain mediated endothelial cell differentiation / migration activities regulation study with this protein as either coating matrix protein or as soluble factor. 2. May be used for KNG1 H chain protein – protein interaction assay. 3. May be used as enzymatic substrate for various proteases. 4. May be used for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFQESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMK Download Datasheet |