Calsyntenins (CLSTN) also known as alcadeins, are type I transmembrane proteins that belong to the cadherin superfamily. Their names come from their ability to bind calcium. CLSTN family consists of three members (CLSTN 1, 2 and 3), CLSTN1 was first shown to be predominantly expressed in postsynaptic membranes of excitatory neurons, CLSTN 2 and 3 were found in an increased manner in inhibitory GABAergic neurons. Calsyntenins interact with numerous proteins such as with kinesin-1 and the APP-linker protein X11L/Mint2, and were shown to have multi-purpose functions both within and outside the nervous system. Shortly after the calsyntenins were discovered, they were found to regulate postsynaptic calcium concentration. Later it was found that another key-function is to link vesicles to kinesin light chain (KLC) and thus to co-determine transport of distinct cargo.

Full-length extracellular domain of human CLSTN3 cDNA (20-847aa, derived from BC111491) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro CLSTN3 mediated neuronal cell differentiation regulation study with this protein as either coating matrix protein or soluble factor.

2. May be used as CLSTN3 protein-protein interaction assay.

3. As antigen for specific antibody production.