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| Cata #: | Name of Product: | Price: |
| HRP-1121 | Recombinant Human IGFBP3 Protein | $150 |
| Product Name: | Recombinant Human IGFBP3 Protein |
| Catalog #: | HRP-1121 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human insulin-like growth factor-binding protein 3 (IGFBP7) gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Full-length mature protein of human IGFBP3 cDNA (27-291aa, Isoform-b, derived from BC000013) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | IGFBP3 (BP_53; IBP53) |
| Accession Number: | NP_000589 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Wu,C., et al., Insulin-like factor binding protein-3 promotes the G1 cell cycle arrest in several cancer cell lines. Gene 512 (1), 127-133 (2013)
Stawerska,R., et al., Ghrelin concentration is correlated with IGF-I/IGFBP-3 molar ratio but not with GH secretion in children with short stature. Neuro Endocrinol. Lett. 33 (4), 412-418 (2012) Coverley,J.A., et al., The effect of phosphorylation by casein kinase 2 on the activity of insulin-like growth factor-binding protein-3. Endocrinology 141 (2), 564-570 (2000) |
| Applications: | 1. May be used for in vitro IGFBP3 mediated IGF1 activity regulation study with this protein as either coating matrix protein or soluble factor. 2. May be used as IGFBP3 protein-protein interaction assay. 3. As enzymatic substrate for various proteases, such as Neutrophil proteases, Cathepsin G and elastase, PSA, et al. 4. As antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFGASSGGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK Download Datasheet |