Human VTN (Vitronectin) is a 478 amino acid protein (1-19 = signal domain), belongs to a member of the pexin family. Vitronectin is an abundant glycoprotein found in serum and the extracellular matrix and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin has been speculated to be involved in homeostasis and tumor malignancy. The publication from Katherine’s paper indicated that full-length recombinant human vitronectin protein benefits long-term human ES cell culture when used as coating matrix protein.

Human mature Vitronectin gene (478 aa) was constructed with codon optimization and expressed in non-fusion protein form in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Coating this recombinant protein at 5-10 µg / well (6 well plate) in xeno-free NutriStem medium can be used for long-term human ES cells maintenance or human iPS cell generation in vitro.